{"id":2967,"date":"2021-04-13T19:43:32","date_gmt":"2021-04-13T17:43:32","guid":{"rendered":"https:\/\/www.agingproject.uniupo.it\/?post_type=pubblicazione&#038;p=2967"},"modified":"2021-04-13T19:43:32","modified_gmt":"2021-04-13T17:43:32","slug":"role-of-combined-lipoic-acid-and-vitamin-d3-on-astrocytes-as-a-way-to-prevent-brain-ageing","status":"publish","type":"pubblicazione","link":"https:\/\/www.agingproject.uniupo.it\/en\/research\/publications\/role-of-combined-lipoic-acid-and-vitamin-d3-on-astrocytes-as-a-way-to-prevent-brain-ageing\/","title":{"rendered":"Role of Combined Lipoic Acid and Vitamin D3 on Astrocytes as a Way to Prevent Brain Ageing"},"content":{"rendered":"<p>Brain aging is a complex multifactorial process characterized by the gradual and continuous loss of neuronal functions. <strong>It is hypothesized that at the basis of brain aging as well as age-related diseases, there is an impairment of the antioxidant defense system leading to an increase of oxidative stress<\/strong>. In this study, two different biological aspects involved in brain aging and neurodegeneration have been investigated: oxidative stress and iron accumulation damage. In primary mouse astrocytes, the stimulation with 50\u2009\u03bcM lipoic acid (LA) and 100\u2009nM vitamin D (vitD) was first investigated in a time-course study to determine the dosages to be used in combination and then in a permeability test using an in vitro blood-brain barrier. In the second set of experiments, the role of oxidative stress was investigated pretreating astrocytes with 200\u2009\u03bcM H2O2 for 30\u2009min. The ability of vitD and LA alone and combined together to prevent or repair the damage caused by oxidative stress was investigated after 24\u2009h of stimulation by the MTT test, mitochondrial membrane potential measurement, and Western blot analysis. To induce neurodegeneration, cells were pretreated with 300\u2009\u03bcM catalytic iron for 6 days and then treated with vitD and LA alone and combined for additional 6 days to investigate the protection exerted by combination, analyzing viability, ROS production, iron concentration, and activation of intracellular pathways. In our study, the combination of LA and vitD showed beneficial effects on the viability of astrocytes, since the substances can cross the brain barrier. In addition, combined LA and vitD attenuated the H2O2-induced apoptosis through the mitochondrial-mediated pathway. The combination was also able to counteract the adverse conditions caused by iron, preventing its accumulation. All these data support the hypothesis of the synergistic and cooperative activity exerted by LA and vitD in astrocytes indicating a possible new strategy to slow down aging.<\/p>\n","protected":false},"template":"","categoria-pubblicazioni":[5054],"class_list":["post-2967","pubblicazione","type-pubblicazione","status-publish","hentry","categoria-pubblicazioni-neuroaging"],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v26.6 (Yoast SEO v26.7) - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Role of Combined Lipoic Acid and Vitamin D3 on Astrocytes as a Way to Prevent Brain Ageing | Aging Project Uniupo<\/title>\n<meta name=\"description\" content=\"Data support hypothesis of the synergistic and cooperative activity exerted by LA and vitD in slowing down brain aging\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.agingproject.uniupo.it\/la-ricerca\/pubblicazioni\/role-of-combined-lipoic-acid-and-vitamin-d3-on-astrocytes-as-a-way-to-prevent-brain-ageing\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Role of Combined Lipoic Acid and Vitamin D3 on Astrocytes as a Way to Prevent Brain Ageing\" \/>\n<meta property=\"og:description\" content=\"Data support hypothesis of the synergistic and cooperative activity exerted by LA and vitD in slowing down brain aging\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.agingproject.uniupo.it\/la-ricerca\/pubblicazioni\/role-of-combined-lipoic-acid-and-vitamin-d3-on-astrocytes-as-a-way-to-prevent-brain-ageing\/\" \/>\n<meta property=\"og:site_name\" content=\"Aging Project Uniupo\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:site\" content=\"@ProjectAging\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.agingproject.uniupo.it\/la-ricerca\/pubblicazioni\/role-of-combined-lipoic-acid-and-vitamin-d3-on-astrocytes-as-a-way-to-prevent-brain-ageing\/\",\"url\":\"https:\/\/www.agingproject.uniupo.it\/la-ricerca\/pubblicazioni\/role-of-combined-lipoic-acid-and-vitamin-d3-on-astrocytes-as-a-way-to-prevent-brain-ageing\/\",\"name\":\"Role of Combined Lipoic Acid and Vitamin D3 on Astrocytes as a Way to Prevent Brain Ageing | Aging Project Uniupo\",\"isPartOf\":{\"@id\":\"https:\/\/www.agingproject.uniupo.it\/#website\"},\"datePublished\":\"2021-04-13T17:43:32+00:00\",\"description\":\"Data support hypothesis of the synergistic and cooperative activity exerted by LA and vitD in slowing down brain aging\",\"breadcrumb\":{\"@id\":\"https:\/\/www.agingproject.uniupo.it\/la-ricerca\/pubblicazioni\/role-of-combined-lipoic-acid-and-vitamin-d3-on-astrocytes-as-a-way-to-prevent-brain-ageing\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/www.agingproject.uniupo.it\/la-ricerca\/pubblicazioni\/role-of-combined-lipoic-acid-and-vitamin-d3-on-astrocytes-as-a-way-to-prevent-brain-ageing\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/www.agingproject.uniupo.it\/la-ricerca\/pubblicazioni\/role-of-combined-lipoic-acid-and-vitamin-d3-on-astrocytes-as-a-way-to-prevent-brain-ageing\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/www.agingproject.uniupo.it\/homepage\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Pubblicazioni\",\"item\":\"https:\/\/www.agingproject.uniupo.it\/la-ricerca\/pubblicazioni\/\"},{\"@type\":\"ListItem\",\"position\":3,\"name\":\"Role of Combined Lipoic Acid and Vitamin D3 on Astrocytes as a Way to Prevent Brain Ageing\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/www.agingproject.uniupo.it\/#website\",\"url\":\"https:\/\/www.agingproject.uniupo.it\/\",\"name\":\"Aging Project Uniupo\",\"description\":\"Facing the challenge of longevity, through interdisciplinary research\",\"publisher\":{\"@id\":\"https:\/\/www.agingproject.uniupo.it\/#organization\"},\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/www.agingproject.uniupo.it\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"},{\"@type\":\"Organization\",\"@id\":\"https:\/\/www.agingproject.uniupo.it\/#organization\",\"name\":\"Aging Project - 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